Southern blot
英 [ˈsʌðən blɒt]
美 [ˈsʌðərn blɑːt]
网络 印迹杂交; Southern印迹法; 核酸杂交技术; 印迹技术; Southern印迹
双语例句
- Technological Improvement of Southern Blot Using Digoxigenin-labeled Probes with Random-primed Method
***随机引物法标记探针的Southern杂交技术优化 - Studied on DIG-labeled Southern Blot Analysis and Cold Resistance Correlation Characteristics in Transgenic Lines of Apple Carrying Exogenous CBF Gene
转CBF基因苹果株系***标记Southern杂交分析及相关抗寒性状研究 - Methods: DNase ⅰ sensitivity assay, Southern blot.
方法：DNaseⅠ敏感性分析法。 - Positive clones with the correct recombination were confirmed by genomic PCR and Southern blot analysis.
以特异性引物经基因组PCR及Southern印迹分析对阳性克隆进行检测。 - PCR and Southern blot demonstrated that the two genes were integrated into the genome of the tobacco plants.
，经PCR扩增和Southernblot分析，证明外源双价目的基因已同时整合到转基因植株的基因组中。 - Methods PCR amplification and southern blot analysis.
方法PCR反应及Southern杂交分析。 - Physical states of 8 HPV 11 DNA were analyzed by techniques of restriction endonuclease and Southern blot hybridization.
用限制性内切酶和Southern转印杂交法分析了8例HPV11DNA的物理状态。 - We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization.
我们用常见的克隆载体pBR322作探针，用核酸点杂交和Southern吸印方法，检测了来自173人的213例样品。 - Results RT-PCR and Southern blot analysis confirmed the integration and expression of tk gene in Hep-2 cells.
结果RT-PCR及Southernblot分析证明tk基因在人喉癌细胞系Hep-2中有整合及表达； - The technique is useful in the analysis of restriction endonuclease digestion of genomic DNA and Southern blot.
该法提取的血吸虫基因组DNA较纯，适合于以后进行DNA限制性核酸内切酶谱分析和Southernblot分析。 - Qualitative Polymerase Chain Reaction ( PCR) and Southern blot are widely used for screening.
定性PCR和Southern印迹是筛选的常用方法。 - Genomic southern blot analysis suggested the existence of two genes at least.
Southern杂交分析显示，在基因组中至少有两个基因存在。 - Integration of the cDNA into the genome of tomato plants was confirmed by Dot blot and Southern blot analysis.
经点杂交和Southern印迹分析，证明部分抗性植株中整合了epo基因。 - Southern blot indicated that the LIF cDNA was stably integrated into the genomic DNA of host cells.
Southernblot检测表明LIFcDNA已经稳固整合到了宿主细胞的基因组DNA中。 - METHODS Use of southern blot hybridization to detect EGFR amplification.
方法采用Southern印迹法观察脑肿瘤中EGFR的扩增。 - Transgenic mice were identified by PCR and Southern blot;
PCR及Southernblot鉴定转基因小鼠基因型； - Southern blot analysis showed that AMS gene is at least three gene copies per genome.
Southernblot分析表明AMS基因在青蒿基因组中至少有3个拷贝。 - Using Southern blot of gene localization, vegetative insecticidal protein gene was determined neither on chromosome nor circle plasmids.
通过Southern杂交的基因定位实验，证实了营养期杀虫蛋白基因并非位于染色体和环型质粒上； - PCR analysis and southern blot hybridization showed primarily that SP gene has been transferred into rice.
分别经PCR和Southern点杂交分析，结果表明目的基因已整合到水稻基因组中。 - Other techniques include nonquantitative or semiquantitative PCR, Southern blot and reporter gene expression ( LacZ, luciferase and GFP).
其它的技术包括非定量或半定量PCR、Southernblot和报告基因表达（LacZ、荧光素酶和绿色荧光蛋白GFP）技术等。 - From tests of hygromycin resistance, PCR and Southern blot, 14 independent transgenic plants were confirmed.
经潮霉素抗性、PCR和Southernblot确证，共获得转基因水稻14株。 - Southern blot analysis showed that there were two copies of the gene in the three accessions.
Southernblot分析结果表明，三种材料中该基因均含有两个拷贝。 - The Southern blot results showed the St-CBF gene had only one allele in potato genome.
Southern杂交显示St-CBF基因在马铃薯基因组中只有一个位点。 - Analysis by PCR and southern blot, seven of them are transgenic.
通过PCR技术和Southern-blot鉴定，获得了七个转基因小鼠系。 - Southern blot analysis was used to determine the exogenous gene integration of PCR positive plants.
为进一步确定外源基因的整合情况，对PCR阳性植株进行Southernblot分析。 - PCR, Southern blot analyses confirmed the transgenic status of some plants.
经PCR、Southernblot分析确认了其中4株独立的转基因植株。 - The results from normal PCR and Southern blot showed T-DNA has inserted into rice genome.
常规PCR和Southernblot结果表明T-DNA区已整合到水稻基因组中。